4RXF
Fructose-6-phosphate aldolase Y131F from E.coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91844 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 88.400, 133.602, 101.590 |
Unit cell angles | 90.00, 92.50, 90.00 |
Refinement procedure
Resolution | 101.490 - 2.400 |
R-factor | 0.19595 |
Rwork | 0.194 |
R-free | 0.23470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l6w |
RMSD bond length | 0.008 |
RMSD bond angle | 1.224 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 101.490 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.105 | 0.465 |
Number of reflections | 91562 | |
<I/σ(I)> | 12.7 | 3.7 |
Completeness [%] | 99.5 | 98.7 |
Redundancy | 5.8 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.2 | 19% PEG3350, 0.1M HEPES pH 8, 0.2M LiCl, VAPOR DIFFUSION, HANGING DROP, temperature 293.2K |