4RX1
Crystal Structure of antibiotic-resistance methyltransferase Kmr
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 193 |
Detector technology | CCD |
Collection date | 2011-01-11 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.54 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 89.506, 89.506, 127.875 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.350 - 2.472 |
R-factor | 0.2162 |
Rwork | 0.214 |
R-free | 0.26310 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.397 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.470 |
Number of reflections | 21690 |
Completeness [%] | 99.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 277 | 100 mM MES buffer pH 6.1, 6% PEG MME 5000, 8% 1-propanol, 0.5 mM SAM, 100 mM sodium iodide, VAPOR DIFFUSION, SITTING DROP, temperature 277K |