4RWW
Crystal Structure of L. monocytogenes PstA in complex with cyclic-di-AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.108, 51.788, 52.996 |
| Unit cell angles | 110.80, 105.59, 109.84 |
Refinement procedure
| Resolution | 29.580 - 1.600 |
| R-factor | 0.15909 |
| Rwork | 0.157 |
| R-free | 0.19446 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m05 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.693 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.090 | 0.416 |
| Number of reflections | 54378 | |
| <I/σ(I)> | 14.7 | 3.2 |
| Completeness [%] | 96.6 | 94.6 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 298 | pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
