4RWT
Structure of actin-Lmod complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2014-07-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.350, 65.650, 81.920 |
| Unit cell angles | 101.29, 90.94, 107.97 |
Refinement procedure
| Resolution | 51.070 - 2.980 |
| R-factor | 0.24865 |
| Rwork | 0.248 |
| R-free | 0.25720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.289 |
| Data reduction software | MOSFLM |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.070 | 3.078 |
| High resolution limit [Å] | 2.980 | 3.000 |
| Number of reflections | 25186 | |
| Completeness [%] | 97.5 | 97.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 20% PGE3350, 0.2M Magnesium acetate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
