4RWQ

Crystal structure of the apo-state of porcine OAS1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-22
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.87260
Spacegroup name	C 1 2 1
Unit cell lengths	79.840, 145.080, 80.140
Unit cell angles	90.00, 106.40, 90.00

Refinement procedure

Resolution	45.498 - 3.100
R-factor	0.2121
Rwork	0.210
R-free	0.26040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.660
Data reduction software	XDS
Data scaling software	SADABS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.000
High resolution limit [Å]	3.100
Number of reflections	15919
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	0.2 M (NH4)2SO4, 0.1 M Sodium acetate pH 5.5, 10% PEG 2000 MME, VAPOR DIFFUSION, SITTING DROP, temperature 291K