4RWG
Crystal structure of the CLR:RAMP1 extracellular domain heterodimer with bound high affinity CGRP analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2014-11-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 172.806, 104.623, 136.483 |
| Unit cell angles | 90.00, 122.43, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.440 |
| R-factor | 0.2019 |
| Rwork | 0.200 |
| R-free | 0.24260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3C4M and 3N7S |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.516 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.440 | 6.650 | 2.450 |
| Rmerge | 0.058 | 0.033 | 0.647 |
| Number of reflections | 76012 | ||
| <I/σ(I)> | 10.8 | ||
| Completeness [%] | 99.8 | 98.6 | 98.6 |
| Redundancy | 4.2 | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 16% PEG3350, 8% Tacsimate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
