4RVZ
Crystal structure of tRNA fluorescent labeling enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2013-07-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 69.623, 69.623, 211.269 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.066 - 2.900 |
| R-factor | 0.214 |
| Rwork | 0.210 |
| R-free | 0.29190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | selenomethionine derived Tias coordinate |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.263 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.426 | |
| Number of reflections | 11960 | |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 50 mM HEPES, pH 7.0-7.2, 0.5 M NH4Ac, 0.02 M MgCl2, 1.5-2.5% PEG8000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
