4RUF
Human K2P4.1 (TRAAAK) potassium channel, W262S mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-06 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03320 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.053, 127.982, 130.406 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.993 - 3.400 |
R-factor | 0.2988 |
Rwork | 0.297 |
R-free | 0.32830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4i9w |
RMSD bond length | 0.003 |
RMSD bond angle | 0.852 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.310 | 3.670 |
High resolution limit [Å] | 3.400 | 3.400 |
Number of reflections | 38349 | |
Completeness [%] | 100.0 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277.15 | 24% PEG400, 10mM Sarcosine, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |