4RTK
Complex of Escherichia coli DNA Adenine Methyltransferase (DAM) with SAH and with DNA Containing Distal Pap Regulon Sequence
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-15 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.331, 63.044, 142.185 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.200 - 1.960 |
R-factor | 0.211 |
Rwork | 0.210 |
R-free | 0.22330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g1p |
RMSD bond length | 0.002 |
RMSD bond angle | 0.600 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | GLRF |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.222 | 2.030 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.057 | 0.297 |
Number of reflections | 23847 | |
<I/σ(I)> | 20.4 | 4.5 |
Completeness [%] | 98.0 | 99.6 |
Redundancy | 4.3 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 289 | 16% PEG200, 100mM KCl, 10mM MgSO4, 100mM MES buffer, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 289K |