4RTJ
A non-cognate complex of Escherichia coli DNA Adenine Methyltransferase (DAM) with DNA and Sinefungin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.367, 63.114, 145.043 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.500 - 1.990 |
| R-factor | 0.215 |
| Rwork | 0.214 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g1p |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.585 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | GLRF |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.509 | 2.060 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.051 | 0.374 |
| Number of reflections | 23469 | |
| <I/σ(I)> | 20.9 | 5.5 |
| Completeness [%] | 98.5 | 98 |
| Redundancy | 7.7 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 289 | 18% PEG200, 100mM KCl, 10mM MgSO4, 100mM MES buffer, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
