4RTB
X-ray structure of the FeFe-hydrogenase maturase HydG from Carboxydothermus hydrogenoformans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-13 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 92.230, 92.230, 167.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.780 - 2.790 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.25500 |
| Structure solution method | AND SAD |
| Starting model (for MR) | 1r30 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.819 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.784 |
| High resolution limit [Å] | 2.789 |
| Number of reflections | 34198 |
| Completeness [%] | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 298 | Ammonium Sulfate 1M, HEPES 100 mM pH 7.5, 0.5% PEG 8000 , VAPOR DIFFUSION, HANGING DROP, temperature 298K |
