4RT4

Crystal structure of Dpy30 complexed with Bre2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.999
Spacegroup name	P 61
Unit cell lengths	75.229, 75.229, 105.758
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.440 - 1.997
R-factor	0.1822
Rwork	0.180
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3g36
RMSD bond length	0.007
RMSD bond angle	1.043
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	2.710	2.050
High resolution limit [Å]	1.997	2.580	2.000
Rmerge	0.069	0.120	0.396
Number of reflections	23016
<I/σ(I)>	35.1	18.3	2.4
Completeness [%]	99.9	100	100
Redundancy	11.3	11.4	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291	0.1M Tris-HCl, pH 8.5, 2.0M NH4H2PO4, EVAPORATION, temperature 291K