4RS8

Apo structure of novel pNOB8 plasmid centromere binding protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979, 0.980, 0.965
Spacegroup name	P 21 21 21
Unit cell lengths	34.300, 63.600, 86.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.150 - 2.290
R-factor	0.2144
Rwork	0.214
R-free	0.25840
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	0.995
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.150
High resolution limit [Å]	2.290	2.290
Number of reflections	15804
Completeness [%]	96.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	30% PEG1500, phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K