4RS6
Crystal structure of the C domain of Polo like Kinase II in Homo Sapiens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 153.075, 153.075, 153.075 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.830 - 2.600 |
| R-factor | 0.18891 |
| Rwork | 0.186 |
| R-free | 0.24724 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j7b |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.737 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 7.010 | 2.600 |
| Rmerge | 0.160 | 0.150 | 0.860 |
| Number of reflections | 17518 | ||
| <I/σ(I)> | 27 | 1.5 | |
| Completeness [%] | 88.0 | 100 | 100 |
| Redundancy | 21.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 35%(v/v) Tacsimate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
