4RS5
Crystal structure of an uncoating intermediate of a EV71 recombinant virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2013-11-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.281579 |
| Spacegroup name | P 42 3 2 |
| Unit cell lengths | 350.155, 350.155, 350.155 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.791 - 3.805 |
| R-factor | 0.2714 |
| Rwork | 0.271 |
| R-free | 0.29940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.981 |
| RMSD bond angle | 0.005 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.791 | 3.940 |
| High resolution limit [Å] | 3.805 | 3.800 |
| Number of reflections | 97564 | |
| Completeness [%] | 81.9 | 94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 290 | 0.1M MES containing 1.6M sodium acetate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
