4RR3

Crystal structure of a recombinant EV71 virus particle

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	90
Detector technology	CCD
Collection date	2012-06-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.978030
Spacegroup name	P 42 3 2
Unit cell lengths	350.599, 350.599, 350.599
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.438 - 3.103
R-factor	0.2358
Rwork	0.235
R-free	0.26520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	1.131
RMSD bond angle	0.006
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.150
High resolution limit [Å]	3.100	3.100
Rmerge		0.967
<I/σ(I)>		2.5
Completeness [%]	90.7	100
Redundancy		6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	290	0.1M Imidazole containing 1M sodium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K