4RR2
Crystal structure of human primase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 86.195, 88.899, 94.682 |
| Unit cell angles | 93.82, 96.57, 111.72 |
Refinement procedure
| Resolution | 48.480 - 2.650 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.27100 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.740 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.690 | 2.600 |
| Rmerge | 0.040 | 0.485 | 0.653 |
| Number of reflections | 72404 | ||
| <I/σ(I)> | 27.8 | 2 | 1.53 |
| Completeness [%] | 91.5 | 93.6 | 93.5 |
| Redundancy | 3.8 | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 50 mM Tris-HCl pH 8.5, 7.5% v/v ethanol and 2 mM TCEP, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
