4RQS

Crystal structure of fully glycosylated HIV-1 gp120 core bound to CD4 and 17b Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	171.318, 171.318, 151.857
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.500 - 4.493
R-factor	0.2785
Rwork	0.276
R-free	0.32180
RMSD bond length	0.003
RMSD bond angle	0.757
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.500	4.700
High resolution limit [Å]	4.493	4.493
Number of reflections	13317
<I/σ(I)>		2.4
Completeness [%]	95.2	97.3
Redundancy		4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	1.4 M sodium formate, 16.6% PEG 3350, 0.1 M CaCl2, 0.1 M Acetate pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K