4RQE
human Seryl-tRNA synthetase dimer complexed with two molecules of tRNAsec
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.809, 142.023, 115.456 |
| Unit cell angles | 90.00, 101.95, 90.00 |
Refinement procedure
| Resolution | 36.394 - 4.000 |
| R-factor | 0.2759 |
| Rwork | 0.274 |
| R-free | 0.32200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4l87 3a3a |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.696 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.140 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.085 | 0.759 |
| Number of reflections | 15058 | |
| <I/σ(I)> | 16.2 | 2.2 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | 18%(m/v) PEG3350, 0.1M NaCl, 0.1M Tris-HCl (pH8.0), 0.1M Sodium malonate pH7.0. , VAPOR DIFFUSION, temperature 298K |
