4RPT
The 1.35A structure of a viral RNase L antagonist reveals basis for the 2'-5'-oligoadenylate binding and enzyme activity.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2014-04-28 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.240, 68.370, 52.420 |
| Unit cell angles | 90.00, 106.88, 90.00 |
Refinement procedure
| Resolution | 31.860 - 1.350 |
| R-factor | 0.192 |
| Rwork | 0.191 |
| R-free | 0.20600 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.264 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.860 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.039 | 0.471 |
| Number of reflections | 56612 | |
| <I/σ(I)> | 19.2 | 3.2 |
| Completeness [%] | 95.2 | 93.5 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 298 | 14% PEG3350, 0.2 M sodium malonate, pH 4, 1 M NaI, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
