4RPE

Crystal Structure of Variant G186E from Pseudomonas Aeruginosa Lipoxygenase 2 at 1.60A (C2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9334
Spacegroup name	C 1 2 1
Unit cell lengths	84.630, 96.880, 87.320
Unit cell angles	90.00, 107.22, 90.00

Refinement procedure

Resolution	19.840 - 1.600
R-factor	0.22902
Rwork	0.227
R-free	0.27170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.905
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.840
High resolution limit [Å]	1.600	1.600
Number of reflections	84566
Completeness [%]	95.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K