4RPD

Crystal Structure of P Domain of 485 Norovirus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97924
Spacegroup name	C 1 2 1
Unit cell lengths	99.410, 73.500, 81.810
Unit cell angles	90.00, 94.11, 90.00

Refinement procedure

Resolution	48.840 - 1.510
Rwork	0.146
R-free	0.18700
Structure solution method	AB INITIO PHASING
RMSD bond length	0.009
RMSD bond angle	1.286
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1690))

Data quality characteristics

	Overall
Low resolution limit [Å]	48.840
High resolution limit [Å]	1.500
Rmerge	0.065
Number of reflections	92638
<I/σ(I)>	14.24
Completeness [%]	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1			291	Potassium Formate 0.2 M, 20% w/v PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 18K, temperature 291K