4RP1
Sequence and structure of a self-assembled 3-D DNA crystal: D(GGATACGTAGGAG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-29 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979200 |
Spacegroup name | P 64 |
Unit cell lengths | 40.805, 40.805, 51.668 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.340 - 2.270 |
R-factor | 0.20616 |
Rwork | 0.202 |
R-free | 0.24394 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p1y |
RMSD bond length | 0.007 |
RMSD bond angle | 1.294 |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.340 | 2.400 |
High resolution limit [Å] | 2.270 | 2.270 |
Rmerge | 0.037 | 1.273 |
Number of reflections | 2219 | |
<I/σ(I)> | 16.4 | 1 |
Redundancy | 2.8 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 298 | 120mM Magnesium Formate, 50mM Lithium Chloride, 10% MPD, pH none, EVAPORATION, temperature 298K |