4ROP
Crystal structure of enolase from Synechococcus elongatus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.127092 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 164.282, 164.282, 75.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.780 - 2.050 |
R-factor | 0.20466 |
Rwork | 0.203 |
R-free | 0.23420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e9i |
RMSD bond length | 0.010 |
RMSD bond angle | 1.632 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.800 | 2.100 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.115 | 0.631 |
Rpim | 0.038 | 0.207 |
Number of reflections | 30631 | 2261 |
<I/σ(I)> | 9 | 2.2 |
Completeness [%] | 98.7 | |
Redundancy | 10.1 | |
CC(1/2) | 0.998 | 0.928 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 10-15% PEG200, 150-200 mM calcium acetate, 0.1 M HEPES, pH 7.5 |