4RON
Sequence and structure of a self-assembled 3-D DNA crystal: D(GGACCATGGGGAG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-29 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979200 |
Spacegroup name | P 64 |
Unit cell lengths | 40.719, 40.719, 51.214 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.260 - 2.390 |
R-factor | 0.21499 |
Rwork | 0.211 |
R-free | 0.25245 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p1y |
RMSD bond length | 0.010 |
RMSD bond angle | 1.327 |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.210 | 2.520 |
High resolution limit [Å] | 2.390 | 2.390 |
Rmerge | 0.030 | 0.747 |
Number of reflections | 1953 | |
<I/σ(I)> | 27.1 | 1.8 |
Redundancy | 5 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 298 | 120mM Magnesium Formate, 50mM Lithium Chloride, 10% MPD, pH none, EVAPORATION, temperature 298K |