4ROK
Sequence and structure of a self-assembled 3-D DNA crystal: D(GGACAGCTGGGAG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-29 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979200 |
Spacegroup name | P 64 |
Unit cell lengths | 40.641, 40.641, 51.983 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.200 - 2.160 |
R-factor | 0.21672 |
Rwork | 0.213 |
R-free | 0.24839 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p1y |
RMSD bond length | 0.008 |
RMSD bond angle | 1.566 |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.200 | 2.280 |
High resolution limit [Å] | 2.160 | 2.160 |
Rmerge | 0.046 | 0.372 |
Number of reflections | 2549 | |
<I/σ(I)> | 17.2 | 2.8 |
Redundancy | 3.4 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 298 | 120mM Magnesium Formate, 50mM Lithium Chloride, 10% MPD, pH none, EVAPORATION, temperature 298K |