4RNW
Truncated version of the G303 Circular Permutation of Old Yellow Enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.247, 73.002, 84.742 |
Unit cell angles | 90.00, 101.27, 90.00 |
Refinement procedure
Resolution | 28.980 - 1.552 |
R-factor | 0.1946 |
Rwork | 0.194 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oya |
RMSD bond length | 0.009 |
RMSD bond angle | 1.259 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.980 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.120 | 0.799 |
Number of reflections | 108578 | |
<I/σ(I)> | 13.7 | 1.9 |
Completeness [%] | 98.0 | 96.6 |
Redundancy | 5.8 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 20% PEG 10000, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |