4RNH
PaMorA tandem diguanylate cyclase - phosphodiesterase, c-di-GMP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-13 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97950 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 81.300, 81.300, 128.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 61.700 - 2.450 |
R-factor | 0.1873 |
Rwork | 0.184 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.566 |
Data reduction software | xia2 |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.700 | 2.520 |
High resolution limit [Å] | 2.450 | 2.460 |
Rmerge | 0.107 | 0.746 |
Number of reflections | 18513 | |
<I/σ(I)> | 14.5 | 2.6 |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 8.1 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2M sodium thiocyanate, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K |