4RMP
Crystal structure of allophycocyanin from marine cyanobacterium Phormidium sp. A09DM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-06-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 101.242, 101.242, 193.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.226 - 2.506 |
| R-factor | 0.1617 |
| Rwork | 0.158 |
| R-free | 0.22880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kn1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.648 |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.640 |
| High resolution limit [Å] | 2.506 | 2.506 |
| Rmerge | 0.109 | 0.557 |
| Number of reflections | 13362 | |
| <I/σ(I)> | 22.8 | 5.3 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 10.8 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 288 | 20% (w/v) PEG 6000, 0.1M bicine, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
