4RMM

Crystal Structure of the Q7NVP2_CHRVO protein from Chromobacterium violaceum. Northeast Structural Genomics Consortium Target CvR191

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-08
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97912
Spacegroup name	P 43 2 2
Unit cell lengths	47.065, 47.065, 120.816
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.855 - 2.200
R-factor	0.245
Rwork	0.244
R-free	0.28000
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.649
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX (D/E)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.061	0.618
Number of reflections	13206
<I/σ(I)>	36.8	2.07
Completeness [%]	99.9	99.8
Redundancy	6.5	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	microbatch under parafin oil	7	291	Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5). Crystallization solution: 20% PEG 4000, 0.1M potassium nitrate, 0.1M Bis-Tris propane, pH 7.0, microbatch under parafin oil, temperature 291K