4RMA
Crystal structure of the FERM domain of human ezrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-29 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95369 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.334, 110.579, 66.124 |
| Unit cell angles | 90.00, 101.57, 90.00 |
Refinement procedure
| Resolution | 35.965 - 1.750 |
| R-factor | 0.2082 |
| Rwork | 0.206 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ni2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.345 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_854)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.670 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.070 | 0.701 |
| Number of reflections | 66327 | |
| <I/σ(I)> | 10.9 | 1.9 |
| Completeness [%] | 97.0 | 94.6 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.2M Ammonium sulfate, 0.1M HEPES pH 7.5, 10% isopropanol, 18% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
