4RM9

Crystal structure of human ezrin in space group C2221

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.954
Spacegroup name	C 2 2 21
Unit cell lengths	67.433, 113.517, 111.773
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.236 - 2.000
R-factor	0.2117
Rwork	0.209
R-free	0.25920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2i1j
RMSD bond length	0.003
RMSD bond angle	0.735
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.3_473))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.970	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.118	0.809
Number of reflections	28759
<I/σ(I)>	14.1	3.7
Completeness [%]	98.3	97.7
Redundancy	14	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	0.1M Bis-Tris pH 5.5, 18% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K