4RL4
Crystal structure of GTP cyclohydrolase II from Helicobacter pylori 26695
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-04-24 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 69.283, 69.283, 179.763 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.650 - 2.199 |
| R-factor | 0.2139 |
| Rwork | 0.212 |
| R-free | 0.26048 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bz0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.237 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.650 | 2.240 |
| High resolution limit [Å] | 2.199 | 2.199 |
| Rmerge | 0.071 | 0.281 |
| Number of reflections | 23212 | |
| <I/σ(I)> | 33.1 | 2.3 |
| Completeness [%] | 96.2 | 72.3 |
| Redundancy | 8.8 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M ammonium citrate tribasic, 15% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
