4RKM
Wolinella succinogenes octaheme sulfite reductase MccA, form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.381 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 211.121, 103.042, 233.070 |
| Unit cell angles | 90.00, 98.46, 90.00 |
Refinement procedure
| Resolution | 49.370 - 2.200 |
| R-factor | 0.18546 |
| Rwork | 0.184 |
| R-free | 0.22011 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.309 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.370 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.199 |
| Number of reflections | 500807 |
| <I/σ(I)> | 9.2 |
| Completeness [%] | 100.0 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.25 | 298 | 12% PEG 5000 MME, 0.1M HEPES/NaOH, 5% tacsimate pH 7.0, 4mM Zwittergent 3-12, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
