4RKH
Structure of the MSL2 CXC domain bound with a specific MRE sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2012-11-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97913 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.371, 50.998, 124.934 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.752 - 2.000 |
| R-factor | 0.2002 |
| Rwork | 0.197 |
| R-free | 0.25430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.322 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.118 | 0.620 |
| Number of reflections | 21109 | |
| <I/σ(I)> | 22.1 | |
| Completeness [%] | 94.9 | 83.7 |
| Redundancy | 6.4 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES-Na (pH 7.5), 10% PEG 3350 (w/v), 0.2M proline, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
