4RKG
Structure of the MSL2 CXC domain bound with a non-specific (GC)6 DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2012-10-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97913 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.705, 75.251, 77.436 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.015 - 2.500 |
| R-factor | 0.2306 |
| Rwork | 0.227 |
| R-free | 0.31490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.053 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.550 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.699 | |
| Number of reflections | 8584 | |
| Completeness [%] | 98.3 | 100 |
| Redundancy | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES-Na (pH 7.5), 25% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
