4RJT
Crystal Structure of Unliganded, Full Length hUGDH at pH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2014-03-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.980, 113.960, 97.120 |
| Unit cell angles | 90.00, 116.91, 90.00 |
Refinement procedure
| Resolution | 48.400 - 2.700 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.23050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4QEJ |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.636 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 2.860 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.118 | 1.700 |
| Number of reflections | 47231 | |
| <I/σ(I)> | 19.6 | 1.4 |
| Completeness [%] | 99.3 | 97.4 |
| Redundancy | 7.4 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.05 M Sodium Formate, 15% PEG 3350, 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
