4RJ9

Structure of a plant specific C2 domain protein, OsGAP1 from rice

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2011-05-30
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	37.190, 166.750, 60.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.365 - 1.630
R-factor	0.1847
Rwork	0.183
R-free	0.20390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cm6
RMSD bond length	0.007
RMSD bond angle	1.214
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.370	30.370
High resolution limit [Å]	1.630	1.630
Rmerge	0.106
Number of reflections	23481
Completeness [%]	98.1	98
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1M Potassium sodium tartrate terrahydrate and 20% w/v PEG3350, pH 8.4-9.1, VAPOR DIFFUSION, SITTING DROP, temperature 289K