4RIW
Crystal structure of an EGFR/HER3 kinase domain heterodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.115869 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.780, 154.640, 86.970 |
| Unit cell angles | 90.00, 110.89, 90.00 |
Refinement procedure
| Resolution | 60.476 - 3.100 |
| R-factor | 0.2327 |
| Rwork | 0.227 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2GS6 AND 3KEX |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.982 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.480 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.191 | 0.602 |
| Number of reflections | 28729 | |
| <I/σ(I)> | 5.2 | 2 |
| Completeness [%] | 97.6 | 99.4 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 7 mg/mL protein, 0.1M HEPES pH 7.5, 14% PEG-6000, 0.2M magnesium chloride, 2mM AMP-PNP, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
