4RIF
Landomycin Glycosyltransferase LanGT2, carbasugar substrate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.970, 59.710, 71.990 |
| Unit cell angles | 87.30, 73.97, 64.92 |
Refinement procedure
| Resolution | 50.360 - 1.850 |
| R-factor | 0.1769 |
| Rwork | 0.176 |
| R-free | 0.19820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | unliganded structure |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.360 |
| High resolution limit [Å] | 1.850 |
| Rmerge | 0.072 |
| Number of reflections | 62872 |
| <I/σ(I)> | 7.5 |
| Completeness [%] | 87.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.8 | 293 | 1.3 M sodium citrate 0.1 M HEPES/NaOH, pH 6.8, VAPOR DIFFUSION, temperature 293K |
