4RI9
FAN1 Nuclease bound to 5' phosphorylated p(dT)/3'(dT-dT-dT-dT-dT-dT-dT-dT) double flap DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.865, 211.574, 69.273 |
| Unit cell angles | 90.00, 99.15, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| R-factor | 0.22827 |
| Rwork | 0.227 |
| R-free | 0.26606 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.376 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 80.000 | 80.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 6.250 | 2.900 |
| Rmerge | 0.092 | 0.041 | 0.642 |
| Number of reflections | 38210 | ||
| <I/σ(I)> | 7.2 | ||
| Completeness [%] | 92.1 | 90.3 | 88.5 |
| Redundancy | 3.8 | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG 3350, NaCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
