4RI8
FAN1 Nuclease bound to 5' phosphorylated p(dG)/3'(dT-dT-dT-dT) double flap DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.260, 210.880, 83.220 |
| Unit cell angles | 90.00, 107.61, 90.00 |
Refinement procedure
| Resolution | 70.000 - 2.900 |
| R-factor | 0.22193 |
| Rwork | 0.221 |
| R-free | 0.25476 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.315 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 80.000 | 80.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 6.250 | 2.900 |
| Rmerge | 0.098 | 0.046 | 0.833 |
| Number of reflections | 42278 | ||
| <I/σ(I)> | 6.8 | ||
| Completeness [%] | 93.7 | 90.2 | 94.7 |
| Redundancy | 7.4 | 7.5 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG 3350, NaCl, pH 7.0, hanging drop vapor diffusion, temperature 277K, VAPOR DIFFUSION, HANGING DROP |
