4RH8

Crystal structure of the outer membrane lipopolysaccharide transport protein LptE (RlpB) from Escherichia coli in the tetragonal crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Detector technology	PIXEL
Collection date	2011-03-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	102.460, 102.460, 166.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	87.310 - 2.200
R-factor	0.22717
Rwork	0.225
R-free	0.26176
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nhr
RMSD bond length	0.018
RMSD bond angle	1.998
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0071)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	200.000	2.260
High resolution limit [Å]	2.200	2.200
Rmerge		1.570
Number of reflections	45803
Completeness [%]	100.0	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.8 M sodium formate, 0.1 M Tris/HOAc pH 8.50, 15% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 277K