4RH7
Crystal structure of human cytoplasmic dynein 2 motor domain in complex with ADP.Vi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-20 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 136.030, 487.150, 276.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.600 - 3.410 |
| R-factor | 0.23989 |
| Rwork | 0.237 |
| R-free | 0.28546 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.548 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 56.500 |
| High resolution limit [Å] | 3.400 |
| Number of reflections | 78411 |
| <I/σ(I)> | 7.6 |
| Completeness [%] | 62.2 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | mixing equal volumes of protein (8mg/ml) and reservoir solution (4-6 % PEG 6000 and 0.1 M Tris pH 8.0), VAPOR DIFFUSION, HANGING DROP, temperature 292K |
