4RH0
Spore photoproduct lyase C140A/S76C mutant with bound AdoMet
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-29 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.00001 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.050, 63.000, 143.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.120 - 2.100 |
R-factor | 0.19603 |
Rwork | 0.194 |
R-free | 0.22952 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.941 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.200 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.083 | 0.497 |
Number of reflections | 33635 | |
Completeness [%] | 99.4 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 200 mM lithium sulfate, 100 mM Tris-HCl, 19-27% PEG800, 70 mM HEGA-8, anaerobic conditions, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |