4REX

Crystal structure of the first WW domain of human YAP2 isoform

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-24
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 2
Unit cell lengths	42.673, 43.101, 21.302
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.551 - 1.600
R-factor	0.1785
Rwork	0.178
R-free	0.19420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ho2
RMSD bond length	0.011
RMSD bond angle	1.356
Data scaling software	Aimless (0.2.13)
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.670	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.104	0.582
Number of reflections	5514
<I/σ(I)>	12	3.2
Completeness [%]	99.2	96.9
Redundancy	6.9	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5	293	1.5 M ammonium sulphate, 0.1 M sodium acetate, 100 mM proline, 5 mM NDSB-201 and 5mM MBCD, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K