4REU
Revelation of Endogenously bound Fe2+ ions in the Crystal Structure of Ferritin from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | I 4 |
| Unit cell lengths | 127.998, 127.998, 170.902 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.17506 |
| Rwork | 0.172 |
| R-free | 0.23539 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eum |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.523 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | CCP4 (Molrep) |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.092 | 0.566 |
| Number of reflections | 45052 | |
| <I/σ(I)> | 13.9 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 10mM Mg hexahydrtae, 2.0M Lithium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
