4RED
Crystal structure of human AMPK alpha1 KD-AID with K43A mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.07806 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 119.532, 119.532, 215.639 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.900 - 2.950 |
| R-factor | 0.23312 |
| Rwork | 0.230 |
| R-free | 0.27681 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h6d |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.445 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.060 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.096 | |
| Number of reflections | 16876 | |
| <I/σ(I)> | 32.3 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.5 M HNa2PO4/0.5 M HK2PO4, 0.1 M Tris hydrochloride, pH 8.5, 0.1 M H(NH4)2PO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
