4REA
A Nuclease DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 170 |
| Detector technology | PIXEL |
| Collection date | 2013-04-28 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 31 |
| Unit cell lengths | 100.960, 100.960, 115.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.480 - 3.810 |
| R-factor | 0.2443 |
| Rwork | 0.243 |
| R-free | 0.27690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.546 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.500 | 3.950 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.207 | |
| Number of reflections | 11982 | |
| <I/σ(I)> | 2.6 | 0.1 |
| Completeness [%] | 97.7 | 85.9 |
| Redundancy | 1.4 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | micro-batch under oil | 300 | 0.1-0.2M KSCN, 18-22% PEG 3350, 0.1M BisTris-propane, micro-batch under oil, temperature 300K |
