4RDT
Structure of the bacterial Zn-transporter ZnuD from Neisseria meningitidis (flexible conformation bound to a zinc ion)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-22 |
| Detector | ADSC Q315 detector |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 101.340, 156.227, 158.075 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.928 - 3.200 |
| R-factor | 0.2718 |
| Rwork | 0.270 |
| R-free | 0.30470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.552 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.230 |
| High resolution limit [Å] | 3.200 | 9.300 | 3.190 |
| Rmerge | 0.134 | 0.048 | 0.661 |
| Number of reflections | 50916 | ||
| <I/σ(I)> | 4.8 | ||
| Completeness [%] | 99.9 | 98.9 | 99.8 |
| Redundancy | 8.1 | 6.8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 300 | 0.1M Hepes pH 7.2, 1M Magnesium Sulfate, vapor diffusion, hanging drop, temperature 300K |
